Minimal Linkage Error In Microscopy

Enhancing Super-Resolution Microscopy with Direct Fluorescent Labeling of VHHs

In typical staining experiments, the fluorophore is at some distance from the antigen. While this may not disturb conventional fluorescence microscopy, it can be problematic in advanced super-resolution microscopy. Through direct fluorescent labeling of VHHs (10x smaller than an IgG) via click reaction or maleimide chemistry, the fluorophore is as close as possible to the antigen, thus minimizing the ‘linkage error.