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Manduhai Alfa-Actinin 4 VHH

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  • Manduhai Alfa-Actinin 4 VHH

PRODUCT SPECIFICATION

Recombinant Manduhai alfa-actinin 4 nanobody 64.

Catalogue number: sdAb-ACT4-Nb64/Man.

Background

Alfa-actinin4 is a cytoskeletal protein and actin cross-linker encoded by the ACTN4 gene, and is a member of the spectrin superfamily of cytoskeletal proteins. Together with isoform 1, actinin-4 is found in nearly every nonmuscle cell but also in cancer cells. The N-terminal pair of Calponin-homology domains constitute an actin binding site whereas the spectrin repeats likely form a rod-like domain. It forms homodimers and has been observed to shuttle between nucleus and cytoplasm under certain conditions. Alfa-actinin function is not restricted to cell motility and cytoskeletal organization, but extends to modulation of gene expression, apoptosis and endocytosis. Actinin-4 deficient mice develop proteinurea and glomerular disease

Derivatized Actinin nanobody for click chemistry

The Manduhai Actinin 4 nanobody 64 carries at its carboxy-terminus a para-azido-Phe residue, enzymatically inserted. This residue is the same as natural Phe, except that it carries an azido group in its aromatic ring (-N3). As a result, the nanobody is endowed with a singular reactive group, allowing down stream click chemistry. Reproducible and site-specific labeling becomes standard in this way. Through this modification the antigen binding properties of the nanobody remain unchanged because the carboxy-terminal region of a nanobody is generally not involved in antigen binding.

Source and properties

Alfa-actinin 4 nanobody 64 was raised by immunizing a llama with a protein fragment encompassing actinin 4 amino acids 404-765. Nanobody 64 binds to the actinin fragment with a Kd of 1.2 × 10-8 M.

Availability:

The Manduhai Alfa-actinin 4 nanobody 64 comes with a COOH-terminal para-Azido-Phe residue. Available in 10 µg, 50 µg, 100µg quantities. For bulk amounts, please inquire.

Cross reactivity:

Reactivity of this nanobody with alfa-actinin 4 from species other than human has not been tested.

Storage buffer:

20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1mM DTT.

Stability:

Store at -20°C upon arrival. For long term storage, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.

Product citations:

Please enquire about other actinin 4 nanobodies at info@gulliverbiomed.com

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