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Manduhai Cortactin SH3 domain VHH

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  • Manduhai Cortactin SH3 domain VHH

PRODUCT SPECIFICATION

Recombinant Manduhai anti-human Cortactin SH3 nanobody 2.

Catalogue number: sdAb-CTT SH3-Nb2/Man

Background

Cortactin is a multidomain cytoskeletal protein and a crucial component in cell migration (via Arp2/3) and cancer cell invasion and metastasis. Cortactin is an early constituent of podosomes (immune cells) and invadopodia (cancer cells), structures/organelles used by cells to degrade the extracellular matrix and migrate to a site of infection (immune cells) or escape from a primary tumor (cancer cells). The protein is overexpressed in various types of cancer.

Derivatized Cortactin nanobody for click chemistry

The Manduhai Cortactin SH3 nanobody 2 carries at its carboxy-terminus a para-azido-Phe residue, enzymatically inserted. This residue is the same as natural Phe, except that it carries an azido group in its aromatic ring (-N3). As a result, the nanobody is endowed with a singular reactive group, allowing down stream click chemistry. Reproducible and site-specific labeling becomes standard in this way. Through this modification the antigen binding properties of the nanobody remain unchanged because the carboxy-terminal region of a nanobody is generally not involved in antigen binding.

Source and properties

Cortactin SH3 Nb2 was raised by immunizing a dromedary with full length human His6- and SUMO-tagged cortactin. The nanobody binds to the C-terminal SH3 domain with an approximate affinity of 75 nM (determined by ITC). It does not cross-react with HS-1, a close relative of cortactin. The nanobody was shown to act as an intrabody and perturb invadosome stability. It may prevent interaction with a number of proteins known to bind to the SH3 domain, including WIP.

Availability:

The Manduhai Cortactin SH3 nanobody 2 comes with a COOH-terminal para-Aziodo Phe residue. Available in 25 µg, 50 µg, 100 µg quantities. For bulk amounts, please inquire.

Expression host:

VHH single domain antibody purified from E. coli.

Cross reactivity:

Reactivity of this nanobody with cortactin from other species has not been tested.

Storage buffer:

20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1mM DTT.

Stability:

Store at -20°C upon arrival. For long term storage, aliquot and store at -80°C. Avoid repeated freeze/thaw cycles.
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