{"id":6034,"date":"2024-10-22T13:58:41","date_gmt":"2024-10-22T13:58:41","guid":{"rendered":"https:\/\/explorelogicsit.net\/gulliverbiomed\/?page_id=6034"},"modified":"2024-10-28T05:49:55","modified_gmt":"2024-10-28T05:49:55","slug":"custom-vhhs","status":"publish","type":"page","link":"https:\/\/explorelogicsit.net\/gulliverbiomed\/custom-vhhs\/","title":{"rendered":"Custom VHHs"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"6034\" class=\"elementor elementor-6034\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e6796ec elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e6796ec\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d0cbffd\" data-id=\"d0cbffd\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-499ddef elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"499ddef\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-df27ce8\" data-id=\"df27ce8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7746972 img-box13 elementor-widget elementor-widget-image\" data-id=\"7746972\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"615\" height=\"497\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/erewrw34.png\" class=\"attachment-full size-full wp-image-6428\" alt=\"\" srcset=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/erewrw34.png 615w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/erewrw34-300x242.png 300w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/erewrw34-600x485.png 600w\" sizes=\"(max-width: 615px) 100vw, 615px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-26c7c39\" data-id=\"26c7c39\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\r\n\t\t<div class=\"elementor-element elementor-element-0cc4e83 st-l elementor-widget elementor-widget-drillersectiontitle\" data-id=\"0cc4e83\" data-element_type=\"widget\" data-widget_type=\"drillersectiontitle.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"title-area\" ><span class=\"sub-title6 title-selector justify-content-center\"><span class=\"shape left\"><span class=\"dots\"><\/span><\/span><span class=\"box\"><\/span> VHHs<span class=\"shape right\"><span class=\"dots\"><\/span><\/span><\/span><h2 class=\"sec-title subtitle-selector\">Your own set of custom VHHs.<\/h2><p>Contact us to obtain information on how to proceed. It is very straightforward. Briefly, we obtain your single domain antibodies from an immunized animal. Turnaround time is approximately 3 weeks. The VHHs are ready for prokaryotic expression and you can use them straight away in your experiments.\n\nOnce you obtained single domain antibodies\/VHHs against your target of interest, many new research opportunities arise. Below is a non-exhaustive list showcasing what you can do.<\/p><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-726010b elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"726010b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-1d6e2ac\" data-id=\"1d6e2ac\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5714367 quotation elementor-widget elementor-widget-text-editor\" data-id=\"5714367\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<blockquote class=\"about-quote\"><h2>Generate VHHs against your target(s) of interest and magnify your research potential<\/h2><div class=\"nano2content\"><p>Now you can generate VHHs that matter most to you and to your research. Include this technology into your next grant application or better even, contact us today. You can either provide us with the purified antigen, or we can generate this in collaboration with you. We guide you through the process, keep you up to date and help you further once you obtain your VHHs of interest. Our researchers have more than two decades of experience in applying them in research.<\/p><\/div><\/blockquote>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-4ab84e0 elementor-hidden-desktop elementor-hidden-tablet elementor-hidden-mobile elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4ab84e0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-0f7fde2\" data-id=\"0f7fde2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-49101ea tab-new-1 elementor-widget elementor-widget-drillermission\" data-id=\"49101ea\" data-element_type=\"widget\" data-widget_type=\"drillermission.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"tab-menu2 filter-menu-active\"><button data-filter=\".1\" class=\"active\" type=\"button\">Versatility<\/button><button data-filter=\".2\" class=\"\" type=\"button\">In cells...<\/button><button data-filter=\".3\" class=\"\" type=\"button\">And more<\/button><button data-filter=\".4\" class=\"\" type=\"button\">Imaging<\/button><button data-filter=\".5\" class=\"\" type=\"button\">....<\/button><\/div><div class=\"mission-box-wrap mt-50 filter-active-1\"><div class=\"filter-item 1\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/1520212958405-1.jpeg\" alt=\"1520212958405 1\"  \/><\/div><div class=\"mission-content\"><h3 class=\"mission-title\">Recombinant expression, intrabody, immunomodulation<\/h3><div class=\"checklist style5\"><ul><li>Express and purify recombinant nanobodies in large amounts (10-100 mg, ormore) at\u00a0<strong>relatively low cost<\/strong>\u00a0in bacteria or yeast. You routinely obtain milligram quantities per liter bacterial culture. Now you have your pet antibodies for the next decade, and longer.<\/li><li>Add any\u00a0<strong>tag<\/strong>\u00a0you like. Remember, you have the nanobody cDNA! Depending on the epitope they recognize, they can be useful for Western blotting, pull-down\/immunoprecipitation experiments. No need to repeatedly purchase antibodies. Let bacteria do the work for you.<\/li><li><strong>Use nanobodies like any other cDNA<\/strong>: clone it into an expression vector and transform, transfect, transduce, nucleofect, electroporate\u2026 your nanobody into a prokaryotic host or into eukaryotic cells and modulate properties of the antigen.<\/li><li>Alternatively, you can use\u00a0<strong>protein transduction to introduce<\/strong>\u00a0a recombinant nanobody directly into cells.<\/li><li>Learn the\u00a0<strong>affinity<\/strong>\u00a0of the nanobody-antigen interaction via SPR, isothermal titration calorimettry, Octet platform\u2026.<\/li><li><strong>Epitope mapping<\/strong>\u00a0becomes easy: using antigen deletion fragments, NMR, X-ray crystallography,..<\/li><\/ul><\/div><\/div><\/div><\/div><div class=\"filter-item 2\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/shutterstock_263643107-1.jpg\" alt=\"shutterstock_263643107 1\"  \/><\/div><div class=\"mission-content\"><h3 class=\"mission-title\">Stuff you can do in cells<\/h3><div class=\"checklist style5\"><ul><li>You can\u00a0<strong>tune expression<\/strong>\u00a0of the nanobody to the expression level of the antigen, avoiding unnecessary overexpression. You know exactly how much nanobody is expressed (generally, you don\u2019t know how much of a pharmacological inhibitor enters cells).<\/li><li><strong>Generate stable cell lines<\/strong>\u00a0that express your nanobody of interest in an inducible manner through i.e. lentiviral transduction.<\/li><li><strong>You no longer require to overexpress your protein (antigen) of interest as a GFP fusion protein<\/strong>, which may cause artefacts.<\/li><li><strong>Trace your protein<\/strong>\u00a0of interest in living cells by coupling a nanobody to a fluorescent protein through simple cDNA cloning.<\/li><li><strong>Delocalize\u00a0<\/strong>your antigen to other compartments in a cell (nucleus, mitochondria, peroxisomes, endoplasmic reticulum\u2026)<\/li><\/ul><\/div><\/div><\/div><\/div><div class=\"filter-item 3\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/shutterstock_187253015-1.jpg\" alt=\"shutterstock_187253015 1\"  \/><\/div><div class=\"mission-content\"><h3 class=\"mission-title\">And more...<\/h3><div class=\"checklist style5\"><ul><li>Trigger protein\u00a0<strong>loss of function<\/strong>\u00a0by tagging your nanobody with a subcellular targeting sequence<\/li><li>Eradicate your target protein of interest from cells\u00a0<strong>(protein knock-out<\/strong>) through degradation via the proteasome. As revolutionary as RNAi in 2001.<\/li><li>Use your nanobody as a\u00a0<strong>chaperone<\/strong>\u00a0for co-crystallization studies, to obtain detailed insight into its epitope.<\/li><li>Nanobodies are conducive to\u00a0<strong>protein crystallization<\/strong>. This can be a stepping stone to development of a small compound inhibitor via medicinal chemistry.<\/li><\/ul><\/div><\/div><\/div><\/div><div class=\"filter-item 4\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/shutterstock_110934590-1.jpg\" alt=\"shutterstock_110934590 1\"  \/><\/div><div class=\"mission-content\"><h3 class=\"mission-title\">High end imaging at cellular, organismal level<\/h3><div class=\"checklist style5\"><ul><li>Label your nanobody with a fluorophore for use in (<strong>super-resolution<\/strong>)\u00a0<strong>fluorescence<\/strong>\u00a0microscopy.<\/li><li>Link them to\u00a0<strong>quantum dots or nano-gold<\/strong>\u00a0particles, without jeopardizing nanobody function.<\/li><li>Turn it into a diagnostic or a\u00a0<strong>tracer<\/strong>\u00a0by labeling with\u00a0<sup>99m<\/sup>Tc or other radionuclide<\/li><li>Use your nanobody in combination with proteomics, screen for interaction partners, or loss of interaction partners (<strong>nanobody<\/strong>\u00a0<strong>footprinting<\/strong>). Due to their small size (15 kDa) they yield much less contaminating peptides in mass spectrometry as a conventional antibody.<\/li><li>Share your nanobody with other researchers and formulate new projects and ideas.<\/li><li>Insert unnatural amino acids at any pre-chosen site in their primary structure and endow them with\u00a0<strong>unique chemical reactivities<\/strong>.<\/li><\/ul><\/div><\/div><\/div><\/div><div class=\"filter-item 5\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/image-1116x784-1.png\" alt=\"image 1116x784 1\"  \/><\/div><div class=\"mission-content\"><div class=\"checklist style5\"><ul><li><p><a href=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/contact-us\/\"><em>Contact us for a return on investment offer.<\/em><\/a><\/p><p>Zoom in on your targets of interest, and conceive the next (bio)medical breakthrough<\/p><\/li><\/ul><\/div><\/div><\/div><\/div><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-8a27231 elementor-hidden-desktop elementor-hidden-tablet elementor-hidden-mobile elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8a27231\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6e3ecba\" data-id=\"6e3ecba\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-ebb0321 elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"ebb0321\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-0651250\" data-id=\"0651250\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0e9549c img-box13 elementor-widget elementor-widget-image\" data-id=\"0e9549c\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"615\" height=\"497\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2023\/10\/about_5_1.png\" class=\"attachment-full size-full wp-image-3242\" alt=\"\" srcset=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2023\/10\/about_5_1.png 615w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2023\/10\/about_5_1-600x485.png 600w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2023\/10\/about_5_1-300x242.png 300w\" sizes=\"(max-width: 615px) 100vw, 615px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-ef245fc\" data-id=\"ef245fc\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-823c88c st-l elementor-widget elementor-widget-drillersectiontitle\" data-id=\"823c88c\" data-element_type=\"widget\" data-widget_type=\"drillersectiontitle.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"title-area\" ><span class=\"sub-title6 title-selector justify-content-center\"><span class=\"shape left\"><span class=\"dots\"><\/span><\/span><span class=\"box\"><\/span> VHHs<span class=\"shape right\"><span class=\"dots\"><\/span><\/span><\/span><h2 class=\"sec-title subtitle-selector\">Your own set of custom VHHs.<\/h2><p>Contact us to obtain information on how to proceed. It is very straightforward. Briefly, we obtain your single domain antibodies from an immunized animal. Turnaround time is approximately 3 weeks. The VHHs are ready for prokaryotic expression and you can use them straight away in your experiments.\n\nOnce you obtained single domain antibodies\/VHHs against your target of interest, many new research opportunities arise. Below is a non-exhaustive list showcasing what you can do.<\/p><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-16d4c0f elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"16d4c0f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-2281456\" data-id=\"2281456\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a58ebf2 quotation elementor-widget elementor-widget-text-editor\" data-id=\"a58ebf2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<blockquote class=\"about-quote\"><h2>Generate VHHs against your target(s) of interest and magnify your research potential<\/h2><div class=\"nano2content\"><p>Now you can generate VHHs that matter most to you and to your research. Include this technology into your next grant application or better even, contact us today. You can either provide us with the purified antigen, or we can generate this in collaboration with you. We guide you through the process, keep you up to date and help you further once you obtain your VHHs of interest. Our researchers have more than two decades of experience in applying them in research.<\/p><\/div><\/blockquote>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-651031c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"651031c\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-ecfb6c5\" data-id=\"ecfb6c5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cbc2aa8 tab-new elementor-widget elementor-widget-drillermission\" data-id=\"cbc2aa8\" data-element_type=\"widget\" data-widget_type=\"drillermission.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"tab-menu2 filter-menu-active\"><button data-filter=\".1\" class=\"active\" type=\"button\">Versatility<\/button><button data-filter=\".2\" class=\"\" type=\"button\">In cells...<\/button><button data-filter=\".3\" class=\"\" type=\"button\">And more<\/button><button data-filter=\".4\" class=\"\" type=\"button\">Imaging<\/button><button data-filter=\".5\" class=\"\" type=\"button\">....<\/button><\/div><div class=\"mission-box-wrap mt-50 filter-active-1\"><div class=\"filter-item 1\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/shutterstock_339120512-e1501333583826.jpg\" alt=\"shutterstock_339120512 e1501333583826\"  \/><\/div><div class=\"mission-content\"><h3 class=\"mission-title\">Recombinant expression, intrabody, immunomodulation<\/h3><div class=\"checklist style5\"><ul><li>Express and purify recombinant nanobodies in large amounts (10-100 mg, ormore) at\u00a0<strong>relatively low cost<\/strong>\u00a0in bacteria or yeast. You routinely obtain milligram quantities per liter bacterial culture. Now you have your pet antibodies for the next decade, and longer.<\/li><li>Add any\u00a0<strong>tag<\/strong>\u00a0you like. Remember, you have the nanobody cDNA! Depending on the epitope they recognize, they can be useful for Western blotting, pull-down\/immunoprecipitation experiments. No need to repeatedly purchase antibodies. Let bacteria do the work for you.<\/li><li><strong>Use nanobodies like any other cDNA<\/strong>: clone it into an expression vector and transform, transfect, transduce, nucleofect, electroporate\u2026 your nanobody into a prokaryotic host or into eukaryotic cells and modulate properties of the antigen.<\/li><li>Alternatively, you can use\u00a0<strong>protein transduction to introduce<\/strong>\u00a0a recombinant nanobody directly into cells.<\/li><li>Learn the\u00a0<strong>affinity<\/strong>\u00a0of the nanobody-antigen interaction via SPR, isothermal titration calorimettry, Octet platform\u2026.<\/li><li><strong>Epitope mapping<\/strong>\u00a0becomes easy: using antigen deletion fragments, NMR, X-ray crystallography,..<\/li><\/ul><\/div><\/div><\/div><\/div><div class=\"filter-item 2\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/shutterstock_263643107.jpg\" alt=\"shutterstock_263643107\"  \/><\/div><div class=\"mission-content\"><h3 class=\"mission-title\">Stuff you can do in cells<\/h3><div class=\"checklist style5\"><ul><li>You can\u00a0<strong>tune expression<\/strong>\u00a0of the nanobody to the expression level of the antigen, avoiding unnecessary overexpression. You know exactly how much nanobody is expressed (generally, you don\u2019t know how much of a pharmacological inhibitor enters cells).<\/li><li><strong>Generate stable cell lines<\/strong>\u00a0that express your nanobody of interest in an inducible manner through i.e. lentiviral transduction.<\/li><li><strong>You no longer require to overexpress your protein (antigen) of interest as a GFP fusion protein<\/strong>, which may cause artefacts.<\/li><li><strong>Trace your protein<\/strong>\u00a0of interest in living cells by coupling a nanobody to a fluorescent protein through simple cDNA cloning.<\/li><li><strong>Delocalize\u00a0<\/strong>your antigen to other compartments in a cell (nucleus, mitochondria, peroxisomes, endoplasmic reticulum\u2026)<\/li><\/ul><\/div><\/div><\/div><\/div><div class=\"filter-item 3\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/shutterstock_187253015-1.jpg\" alt=\"shutterstock_187253015 1\"  \/><\/div><div class=\"mission-content\"><h3 class=\"mission-title\">And more<\/h3><div class=\"checklist style5\"><ul><li>Trigger protein\u00a0<strong>loss of function<\/strong>\u00a0by tagging your nanobody with a subcellular targeting sequence<\/li><li>Eradicate your target protein of interest from cells\u00a0<strong>(protein knock-out<\/strong>) through degradation via the proteasome. As revolutionary as RNAi in 2001.<\/li><li>Use your nanobody as a\u00a0<strong>chaperone<\/strong>\u00a0for co-crystallization studies, to obtain detailed insight into its epitope.<\/li><li>Nanobodies are conducive to\u00a0<strong>protein crystallization<\/strong>. This can be a stepping stone to development of a small compound inhibitor via medicinal chemistry.<\/li><\/ul><\/div><\/div><\/div><\/div><div class=\"filter-item 4\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/shutterstock_110934590.jpg\" alt=\"shutterstock_110934590\"  \/><\/div><div class=\"mission-content\"><h3 class=\"mission-title\">High end imaging at cellular, organismal level<\/h3><div class=\"checklist style5\"><ul><li>Label your nanobody with a fluorophore for use in (<strong>super-resolution<\/strong>)\u00a0<strong>fluorescence<\/strong>\u00a0microscopy.<\/li><li>Link them to\u00a0<strong>quantum dots or nano-gold<\/strong>\u00a0particles, without jeopardizing nanobody function.<\/li><li>Turn it into a diagnostic or a\u00a0<strong>tracer<\/strong>\u00a0by labeling with\u00a0<sup>99m<\/sup>Tc or other radionuclide<\/li><li>Use your nanobody in combination with proteomics, screen for interaction partners, or loss of interaction partners (<strong>nanobody<\/strong>\u00a0<strong>footprinting<\/strong>). Due to their small size (15 kDa) they yield much less contaminating peptides in mass spectrometry as a conventional antibody.<\/li><li>Share your nanobody with other researchers and formulate new projects and ideas.<\/li><li>Insert unnatural amino acids at any pre-chosen site in their primary structure and endow them with\u00a0<strong>unique chemical reactivities<\/strong>.<\/li><\/ul><\/div><\/div><\/div><\/div><div class=\"filter-item 5\"><div class=\"mission-grid\"><div class=\"mission-img rounded-20\" data-overlay=\"title\" data-opacity=\"2\"><img decoding=\"async\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/image-1116x784-1.png\" alt=\"image 1116x784 1\"  \/><\/div><div class=\"mission-content\"><div class=\"checklist style5\"><ul><li><p><a href=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/contact-us\/\"><em>Contact us for a return on investment offer.<\/em><\/a><\/p><p>Zoom in on your targets of interest, and conceive the next (bio)medical breakthrough<\/p><\/li><\/ul><\/div><\/div><\/div><\/div><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c7bb076 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c7bb076\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1309890\" data-id=\"1309890\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-0e8571b elementor-reverse-mobile tab-style-1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0e8571b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-e9bc51e\" data-id=\"e9bc51e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5281556 st-l elementor-widget elementor-widget-drillersectiontitle\" data-id=\"5281556\" data-element_type=\"widget\" data-widget_type=\"drillersectiontitle.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"title-area\" ><span class=\"sub-title6 title-selector justify-content-center\"><span class=\"shape left\"><span class=\"dots\"><\/span><\/span>immunomodulation<span class=\"shape right\"><span class=\"dots\"><\/span><\/span><\/span><h2 class=\"sec-title subtitle-selector\">Recombinant expression, intrabody<\/h2><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c88310e elementor-widget elementor-widget-text-editor\" data-id=\"c88310e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"checklist style5\"><li>Express and purify recombinant nanobodies in large amounts (10-100 mg, ormore) at\u00a0<strong>relatively low cost<\/strong>\u00a0in bacteria or yeast. You routinely obtain milligram quantities per liter bacterial culture. Now you have your pet antibodies for the next decade, and longer.<\/li><li>Add any\u00a0<strong>tag<\/strong>\u00a0you like. Remember, you have the nanobody cDNA! Depending on the epitope they recognize, they can be useful for Western blotting, pull-down\/immunoprecipitation experiments. No need to repeatedly purchase antibodies. Let bacteria do the work for you.<\/li><li><strong>Use nanobodies like any other cDNA<\/strong>: clone it into an expression vector and transform, transfect, transduce, nucleofect, electroporate\u2026 your nanobody into a prokaryotic host or into eukaryotic cells and modulate properties of the antigen.<\/li><li>Alternatively, you can use\u00a0<strong>protein transduction to introduce<\/strong>\u00a0a recombinant nanobody directly into cells.<\/li><li>Learn the\u00a0<strong>affinity<\/strong>\u00a0of the nanobody-antigen interaction via SPR, isothermal titration calorimettry, Octet platform\u2026.<\/li><li><strong>Epitope mapping<\/strong>\u00a0becomes easy: using antigen deletion fragments, NMR, X-ray crystallography,..<\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-910d8a1\" data-id=\"910d8a1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1d06088 elementor-widget elementor-widget-image\" data-id=\"1d06088\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"453\" height=\"670\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/SCSACSACSACSACSAC-1.png\" class=\"attachment-large size-large wp-image-6738\" alt=\"\" srcset=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/SCSACSACSACSACSAC-1.png 453w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/SCSACSACSACSACSAC-1-203x300.png 203w\" sizes=\"(max-width: 453px) 100vw, 453px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-ba334e5 tab-style-1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"ba334e5\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-9241b8f\" data-id=\"9241b8f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0a4459d elementor-widget elementor-widget-image\" data-id=\"0a4459d\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"510\" height=\"500\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/510.png\" class=\"attachment-large size-large wp-image-6739\" alt=\"\" srcset=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/510.png 510w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/510-300x294.png 300w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/510-287x280.png 287w\" sizes=\"(max-width: 510px) 100vw, 510px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-2b196e3\" data-id=\"2b196e3\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a7698ae st-l elementor-widget elementor-widget-drillersectiontitle\" data-id=\"a7698ae\" data-element_type=\"widget\" data-widget_type=\"drillersectiontitle.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"title-area\" ><span class=\"sub-title6 title-selector justify-content-center\"><span class=\"shape left\"><span class=\"dots\"><\/span><\/span>Cell Lines<span class=\"shape right\"><span class=\"dots\"><\/span><\/span><\/span><h2 class=\"sec-title subtitle-selector\">Stuff you can do in cells\n<\/h2><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-da1a9aa elementor-widget elementor-widget-text-editor\" data-id=\"da1a9aa\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"checklist style5\"><li>You can\u00a0<strong>tune expression<\/strong>\u00a0of the nanobody to the expression level of the antigen, avoiding unnecessary overexpression. You know exactly how much nanobody is expressed (generally, you don\u2019t know how much of a pharmacological inhibitor enters cells).<\/li><li><strong>Generate stable cell lines<\/strong>\u00a0that express your nanobody of interest in an inducible manner through i.e. lentiviral transduction.<\/li><li><strong>You no longer require to overexpress your protein (antigen) of interest as a GFP fusion protein<\/strong>, which may cause artefacts.<\/li><li><strong>Trace your protein<\/strong>\u00a0of interest in living cells by coupling a nanobody to a fluorescent protein through simple cDNA cloning.<\/li><li><strong>Delocalize\u00a0<\/strong>your antigen to other compartments in a cell (nucleus, mitochondria, peroxisomes, endoplasmic reticulum\u2026)<\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-c372274 elementor-reverse-mobile tab-style-1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c372274\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-325c742\" data-id=\"325c742\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a003c2a st-l elementor-widget elementor-widget-drillersectiontitle\" data-id=\"a003c2a\" data-element_type=\"widget\" data-widget_type=\"drillersectiontitle.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"title-area\" ><span class=\"sub-title6 title-selector justify-content-center\"><span class=\"shape left\"><span class=\"dots\"><\/span><\/span>loss of function<span class=\"shape right\"><span class=\"dots\"><\/span><\/span><\/span><h2 class=\"sec-title subtitle-selector\">And more...\n<\/h2><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-fc2000d elementor-widget elementor-widget-text-editor\" data-id=\"fc2000d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"checklist style5\"><li>Trigger protein\u00a0<strong>loss of function<\/strong>\u00a0by tagging your nanobody with a subcellular targeting sequence<\/li><li>Eradicate your target protein of interest from cells\u00a0<strong>(protein knock-out<\/strong>) through degradation via the proteasome. As revolutionary as RNAi in 2001.<\/li><li>Use your nanobody as a\u00a0<strong>chaperone<\/strong>\u00a0for co-crystallization studies, to obtain detailed insight into its epitope.<\/li><li>Nanobodies are conducive to\u00a0<strong>protein crystallization<\/strong>. This can be a stepping stone to development of a small compound inhibitor via medicinal chemistry.<\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-75c7ee5\" data-id=\"75c7ee5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c066729 elementor-widget elementor-widget-image\" data-id=\"c066729\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"453\" height=\"400\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/dsfewfewf.png\" class=\"attachment-large size-large wp-image-6740\" alt=\"\" srcset=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/dsfewfewf.png 453w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/dsfewfewf-300x265.png 300w\" sizes=\"(max-width: 453px) 100vw, 453px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-c5d9804 tab-style-1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c5d9804\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-569ceb9\" data-id=\"569ceb9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-38f68cf elementor-widget elementor-widget-image\" data-id=\"38f68cf\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"510\" height=\"670\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/670.png\" class=\"attachment-large size-large wp-image-6741\" alt=\"\" srcset=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/670.png 510w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/670-228x300.png 228w\" sizes=\"(max-width: 510px) 100vw, 510px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-08db90c\" data-id=\"08db90c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-33a4b20 st-l elementor-widget elementor-widget-drillersectiontitle\" data-id=\"33a4b20\" data-element_type=\"widget\" data-widget_type=\"drillersectiontitle.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"title-area\" ><span class=\"sub-title6 title-selector justify-content-center\"><span class=\"shape left\"><span class=\"dots\"><\/span><\/span>super-resolution<span class=\"shape right\"><span class=\"dots\"><\/span><\/span><\/span><h2 class=\"sec-title subtitle-selector\">High end imaging at cellular, organismal level<\/h2><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-db1f81c elementor-widget elementor-widget-text-editor\" data-id=\"db1f81c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"checklist style5\"><li>Label your nanobody with a fluorophore for use in (<strong>super-resolution<\/strong>)\u00a0<strong>fluorescence<\/strong>\u00a0microscopy.<\/li><li>Link them to\u00a0<strong>quantum dots or nano-gold<\/strong>\u00a0particles, without jeopardizing nanobody function.<\/li><li>Turn it into a diagnostic or a\u00a0<strong>tracer<\/strong>\u00a0by labeling with\u00a0<sup>99m<\/sup>Tc or other radionuclide<\/li><li>Use your nanobody in combination with proteomics, screen for interaction partners, or loss of interaction partners (<strong>nanobody<\/strong>\u00a0<strong>footprinting<\/strong>). Due to their small size (15 kDa) they yield much less contaminating peptides in mass spectrometry as a conventional antibody.<\/li><li>Share your nanobody with other researchers and formulate new projects and ideas.<\/li><li>Insert unnatural amino acids at any pre-chosen site in their primary structure and endow them with\u00a0<strong>unique chemical reactivities<\/strong>.<\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-dafe79e elementor-reverse-mobile tab-style-1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"dafe79e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-1e39745\" data-id=\"1e39745\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0c4c1d9 st-l elementor-widget elementor-widget-drillersectiontitle\" data-id=\"0c4c1d9\" data-element_type=\"widget\" data-widget_type=\"drillersectiontitle.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"title-area\" ><h2 class=\"sec-title subtitle-selector\">\n<\/h2><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a2aa916 elementor-widget elementor-widget-text-editor\" data-id=\"a2aa916\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul class=\"checklist style5\"><li><p><a href=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/contact-us\/\"><em>Contact us for a return on investment offer.<\/em><\/a><\/p><p>Zoom in on your targets of interest, and conceive the next (bio)medical breakthrough<\/p><\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-edf3208\" data-id=\"edf3208\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-22eaa4d elementor-widget elementor-widget-image\" data-id=\"22eaa4d\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"453\" height=\"400\" src=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/fefefr3re.png\" class=\"attachment-large size-large wp-image-6742\" alt=\"\" srcset=\"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/fefefr3re.png 453w, https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-content\/uploads\/2024\/10\/fefefr3re-300x265.png 300w\" sizes=\"(max-width: 453px) 100vw, 453px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>VHHs Your own set of custom VHHs. Contact us to obtain information on how to proceed. It is very straightforward. Briefly, we obtain your single domain antibodies from an immunized animal. Turnaround time is approximately 3 weeks. The VHHs are ready for prokaryotic expression and you can use them straight away in your experiments. Once&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-builder.php","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-6034","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6034","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/comments?post=6034"}],"version-history":[{"count":160,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6034\/revisions"}],"predecessor-version":[{"id":6832,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6034\/revisions\/6832"}],"wp:attachment":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/media?parent=6034"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}