{"id":6323,"date":"2024-10-23T07:42:46","date_gmt":"2024-10-23T07:42:46","guid":{"rendered":"https:\/\/explorelogicsit.net\/gulliverbiomed\/?page_id=6323"},"modified":"2024-10-23T11:34:53","modified_gmt":"2024-10-23T11:34:53","slug":"manduhai-alfa-actinin-4-vhh","status":"publish","type":"page","link":"https:\/\/explorelogicsit.net\/gulliverbiomed\/manduhai-alfa-actinin-4-vhh\/","title":{"rendered":"Manduhai Alfa-Actinin 4 VHH"},"content":{"rendered":"\t\t
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PRODUCT SPECIFICATION<\/h2><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Recombinant Manduhai alfa-actinin 4 nanobody 64.<\/p>

Catalogue number:<\/strong> sdAb-ACT4-Nb64\/Man.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Background \n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\tAlfa-actinin4 is a cytoskeletal protein and actin cross-linker encoded by the ACTN4 gene, and is a member of the spectrin superfamily of cytoskeletal proteins. Together with isoform 1, actinin-4 is found in nearly every nonmuscle cell but also in cancer cells. The N-terminal pair of Calponin-homology domains constitute an actin binding site whereas the spectrin repeats likely form a rod-like domain. It forms homodimers and has been observed to shuttle between nucleus and cytoplasm under certain conditions. Alfa-actinin function is not restricted to cell motility and cytoskeletal organization, but extends to modulation of gene expression, apoptosis and endocytosis. Actinin-4 deficient mice develop proteinurea and glomerular disease\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Derivatized Actinin nanobody for click chemistry<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\tThe Manduhai Actinin 4 nanobody 64 carries at its carboxy-terminus a para-azido-Phe residue, enzymatically\ninserted. This residue is the same as natural Phe, except that it carries an azido group in its aromatic ring (-N3).\nAs a result, the nanobody is endowed with a singular reactive group, allowing down stream click chemistry.\nReproducible and site-specific labeling becomes standard in this way. Through this modification the antigen\nbinding properties of the nanobody remain unchanged because the carboxy-terminal region of a nanobody is\ngenerally not involved in antigen binding. \t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Source and properties\n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\tAlfa-actinin 4 nanobody 64 was raised by immunizing a llama with a protein fragment encompassing actinin 4\namino acids 404-765. Nanobody 64 binds to the actinin fragment with a Kd of 1.2 \u00d7 10-8 M.\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Availability: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tThe Manduhai Alfa-actinin 4 nanobody 64 comes with a COOH-terminal para-Azido-Phe residue. Available in 10 \u00b5g, 50 \u00b5g, 100\u00b5g quantities. For bulk amounts, please inquire.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Cross reactivity: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tReactivity of this nanobody with alfa-actinin 4 from species other than human has not been tested.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Storage buffer: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\t20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1mM DTT.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Stability: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tStore at -20\u00b0C upon arrival. For long term storage, aliquot and store at -80\u00b0C. Avoid\nrepeated freeze\/thaw cycles.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Product citations:<\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Please enquire about other actinin 4 nanobodies at info@gulliverbiomed.com<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Read More<\/i><\/a><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

PRODUCT SPECIFICATION Recombinant Manduhai alfa-actinin 4 nanobody 64. Catalogue number: sdAb-ACT4-Nb64\/Man. Background Alfa-actinin4 is a cytoskeletal protein and actin cross-linker encoded by the ACTN4 gene, and is a member of the spectrin superfamily of cytoskeletal proteins. Together with isoform 1, actinin-4 is found in nearly every nonmuscle cell but also in cancer cells. The N-terminal…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-builder.php","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-6323","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/comments?post=6323"}],"version-history":[{"count":47,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6323\/revisions"}],"predecessor-version":[{"id":6522,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6323\/revisions\/6522"}],"wp:attachment":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/media?parent=6323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}