{"id":6409,"date":"2024-10-23T10:31:40","date_gmt":"2024-10-23T10:31:40","guid":{"rendered":"https:\/\/explorelogicsit.net\/gulliverbiomed\/?page_id=6409"},"modified":"2024-10-24T08:19:46","modified_gmt":"2024-10-24T08:19:46","slug":"manduhai-l-plastin-5-9-vhh","status":"publish","type":"page","link":"https:\/\/explorelogicsit.net\/gulliverbiomed\/manduhai-l-plastin-5-9-vhh\/","title":{"rendered":"Manduhai L-plastin 5\/9 VHH"},"content":{"rendered":"\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t
\n\t\t\r\n\t\t
\n\t\t\t\t
\n\t\t\t

PRODUCT SPECIFICATION<\/h2><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t

Recombinant Manduhai anti-human L-plastin nanobody 5 and 9.<\/p>

Catalogue number:<\/strong> sdAb-Plastin-Nb5-9\/Man<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

\n\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t

Background \n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\tL-plastin is member of a family of actin bundling proteins. Unlike fascin, L-plastin generates actin bundles that are more loosely connected. L-plastin expression is normally restricted to immune cells but is ectopically expressed in tumor cells and contributes to tumor cell invasion.\nNanobody 5 and 9 affect filopodium formation, cell motility, perturb stability of immune cell podosomes and cancer cell invadosomes. \t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
\n\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t

Derivatized L-plastin nanobody for click chemistry\n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\tThe Manduhai plastin Nanobodies carry a carboxy-terminal para-azido-Phe residue, enzymatically inserted. This residue is the same as natural Phe, except that it carries an azido group in its aromatic ring (-N3). As a result, the nanobody is endowed with a singular reactive group, allowing down stream click chemistry. Reproducible and site-specific labeling becomes standard in this way. Through this modification the antigen binding properties of the nanobody remain unchanged because the carboxy-terminal region of a nanobody is generally not involved in antigen binding. \t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
\n\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t

Source and properties\n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t

L-plastin nanobody 5 and 9 were raised by immunizing an alpaca with full length human recombinant L-plastin. It binds to L-plastin with an approximate affinity of 80 nM (determined by ITC)<\/strong>. L-plastin nanobody 5 interacts with the combined actin binding domains in Lplasti; nanobody 9 interacts with the EF-hands, in the Nterminal region of L-plastin.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

\n\t\t\t\t\t
\n\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t

Availability: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\tManduhai Nanobody 5 and 9 come with a COOH-terminal para-Azido-Phe residue.\nAvailable in 25 \u00b5g, 50 \u00b5g, 100 \u00b5g quantities. For bulk amounts, please inquire.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t

Expression host:<\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\tVHH single domain antibody purified from E. coli.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t

Cross reactivity: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\tReactivity of this nanobody with L-plastin from other species has not been tested.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t

Storage buffer:<\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1mM DTT\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t

Stability: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\tStore at -20\u00b0C upon arrival. For long term storage, aliquot and store at -80\u00b0C. Avoid\nrepeated freeze\/thaw cycles.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t

Product citations:<\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

\n\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t
  1. Van Audenhove I, Denert M, Boucherie C, et al. 2016. J Biol Chem. 291, 9148-60.<\/li>
  2. De Clercq S, Boucherie C, Vandekerckhove J, Gettemans J, Guillabert A. 2013. PLoS One 8: e78108<\/li>
  3. De Clercq S, Zwaenepoel O, Martens E, Vandekerckhove J, Guillabert A, Gettemans J. 2013. Cell Mol Life Sci.70, 909-22.<\/li><\/ol>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
    \n\t\t\t\t
    \n\t\t\t
    Read More<\/i><\/a><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

    PRODUCT SPECIFICATION Recombinant Manduhai anti-human L-plastin nanobody 5 and 9. Catalogue number: sdAb-Plastin-Nb5-9\/Man Background L-plastin is member of a family of actin bundling proteins. Unlike fascin, L-plastin generates actin bundles that are more loosely connected. L-plastin expression is normally restricted to immune cells but is ectopically expressed in tumor cells and contributes to tumor cell…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-builder.php","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-6409","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6409","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/comments?post=6409"}],"version-history":[{"count":13,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6409\/revisions"}],"predecessor-version":[{"id":6734,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6409\/revisions\/6734"}],"wp:attachment":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/media?parent=6409"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}