{"id":6421,"date":"2024-10-23T10:48:31","date_gmt":"2024-10-23T10:48:31","guid":{"rendered":"https:\/\/explorelogicsit.net\/gulliverbiomed\/?page_id=6421"},"modified":"2024-10-23T10:55:03","modified_gmt":"2024-10-23T10:55:03","slug":"manduhai-cortactin-sh3-domain-vhh","status":"publish","type":"page","link":"https:\/\/explorelogicsit.net\/gulliverbiomed\/manduhai-cortactin-sh3-domain-vhh\/","title":{"rendered":"Manduhai Cortactin SH3 domain VHH"},"content":{"rendered":"\t\t
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PRODUCT SPECIFICATION<\/h2><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Recombinant Manduhai anti-human Cortactin SH3 nanobody 2.<\/p>

Catalogue number:<\/strong> sdAb-CTT SH3-Nb2\/Man<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Background \n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\tCortactin is a multidomain cytoskeletal protein and a crucial component in cell migration (via Arp2\/3) and cancer cell invasion and metastasis. Cortactin is an early constituent of podosomes (immune cells) and invadopodia (cancer cells), structures\/organelles used by cells to degrade the extracellular matrix and migrate to a site of infection (immune cells) or escape from a primary tumor (cancer cells). The protein is overexpressed in various types of cancer. \t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Derivatized Cortactin nanobody for click chemistry\n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\tThe Manduhai Cortactin SH3 nanobody 2 carries at its carboxy-terminus a para-azido-Phe residue, enzymatically inserted. This residue is the same as natural Phe, except that it carries an azido group in its aromatic ring (-N3). As a result, the nanobody is endowed with a singular reactive group, allowing down stream click chemistry. Reproducible and site-specific labeling becomes standard in this way. Through this modification\nthe antigen binding properties of the nanobody remain unchanged because the carboxy-terminal region of a nanobody is generally not involved in antigen binding. \n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Source and properties\n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Cortactin SH3 Nb2 was raised by immunizing a dromedary with full length human His6- and SUMO-tagged cortactin. The nanobody binds to the C-terminal SH3 domain with an approximate affinity of 75 nM (determined by ITC)<\/strong>. It does not cross-react with HS-1, a close relative of cortactin. The nanobody was shown to act as an intrabody and perturb invadosome stability. It may prevent interaction with a number of proteins known to bind to the SH3 domain, including WIP.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Availability: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tThe Manduhai Cortactin SH3 nanobody 2 comes with a COOH-terminal para-Aziodo Phe\nresidue. Available in 25 \u00b5g, 50 \u00b5g, 100 \u00b5g quantities. For bulk amounts, please inquire.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Expression host:<\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tVHH single domain antibody purified from E. coli.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Cross reactivity: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tReactivity of this nanobody with cortactin from other species has not been tested.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Storage buffer:<\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\t20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1mM DTT. \t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Stability: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tStore at -20\u00b0C upon arrival. For long term storage, aliquot and store at -80\u00b0C. Avoid repeated freeze\/thaw cycles.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Read More<\/i><\/a><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

PRODUCT SPECIFICATION Recombinant Manduhai anti-human Cortactin SH3 nanobody 2. Catalogue number: sdAb-CTT SH3-Nb2\/Man Background Cortactin is a multidomain cytoskeletal protein and a crucial component in cell migration (via Arp2\/3) and cancer cell invasion and metastasis. Cortactin is an early constituent of podosomes (immune cells) and invadopodia (cancer cells), structures\/organelles used by cells to degrade the…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-builder.php","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-6421","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6421","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/comments?post=6421"}],"version-history":[{"count":4,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6421\/revisions"}],"predecessor-version":[{"id":6425,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6421\/revisions\/6425"}],"wp:attachment":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/media?parent=6421"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}