{"id":6465,"date":"2024-10-23T11:18:37","date_gmt":"2024-10-23T11:18:37","guid":{"rendered":"https:\/\/explorelogicsit.net\/gulliverbiomed\/?page_id=6465"},"modified":"2024-10-23T11:23:46","modified_gmt":"2024-10-23T11:23:46","slug":"manduhai-sars-cov-2-vhhs","status":"publish","type":"page","link":"https:\/\/explorelogicsit.net\/gulliverbiomed\/manduhai-sars-cov-2-vhhs\/","title":{"rendered":"Manduhai SARS-CoV-2 VHHs"},"content":{"rendered":"\t\t
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PRODUCT SPECIFICATION<\/h2><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\tRecombinant SARS-CoV-2 nanobodies\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Background \n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\tTo maximally meet the challenge of the current corona virus pandemic we need as many tools as possible. Llamas, being out bread animals, are ideally suited for this purpose. Each immunized animal will generate a set of different Nanobodies that bind to different regions of for instance the corona Spike protein, currently one of the major therapeutic targets. As such Nanobodies act as a molecular stethoscope, able of identifying \u2018sensitive epitopes\u2019 in the target protein. They can be further developed into research tools, diagnostics or therapeutics.\n

\nGulliver Biomed has raised dozens of anti Spike protein- and receptor binding domain-specific Nanobodies that can assist in developing the most appropriate strategy to understand and challenge the corona virus pandemic at the molecular level.\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Derivatized Spike and receptor binding domain nanobodies for click chemistry\n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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The \u2018corona Nanobodies\u2019 can be derivatized in such a way that they carry a C-terminal para-azido-Phe residue, or at any other pre-chosen site in their primary structure. This residue is the same as natural Phe, except that it carries an azido group in its aromatic ring (-N3). As a result, the nanobody is endowed with a singular reactive group, allowing down stream click chemistry. Reproducible and site-specific labeling becomes standard in this way. Through this modification the antigen binding properties of the nanobody remain unchanged because the carboxy-terminal region of a nanobody is generally not involved in antigen binding.


Such derivatized Nanobodies are ideal for oriented coupling as in for instance lateral flow immunological assays (LFIA), dramatically increasing sensitivity which is a prerequisite for development of a point-of care device, which can be used anywhere, yielding fast results.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Source and properties\n<\/h4>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\n\t\t\t\t\t\t\tThese Nanobodies were raised by immunizing a llama with the spike protein or the receptor binding domain of Spike.\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Availability: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tManduhai corona virus Nanobodies comes with a COOH-terminal para-Azido-Phe\nresidue. Available in 10 \u00b5g, 50 \u00b5g, 100\u00b5g quantities. For bulk amounts, please inquire\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Storage buffer:<\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\t20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1mM DTT. \t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Stability: <\/p>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\t\t\t\tStore at -20\u00b0C upon arrival. For long term storage, aliquot and store at -80\u00b0C. Avoid\nrepeated freeze\/thaw cycles.\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Read More<\/i><\/a><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"

PRODUCT SPECIFICATION Recombinant SARS-CoV-2 nanobodies Background To maximally meet the challenge of the current corona virus pandemic we need as many tools as possible. Llamas, being out bread animals, are ideally suited for this purpose. Each immunized animal will generate a set of different Nanobodies that bind to different regions of for instance the corona…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"template-builder.php","meta":{"inline_featured_image":false,"footnotes":""},"class_list":["post-6465","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6465","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/comments?post=6465"}],"version-history":[{"count":4,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6465\/revisions"}],"predecessor-version":[{"id":6484,"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/pages\/6465\/revisions\/6484"}],"wp:attachment":[{"href":"https:\/\/explorelogicsit.net\/gulliverbiomed\/wp-json\/wp\/v2\/media?parent=6465"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}